Search results for "SOIL DNA EXTRACTION"
showing 3 items of 3 documents
Biogeographical patterns of soil molecular microbial biomass
2011
Affiche - résumé. Session GC: Microbes in the Changing Environmenl: Global Climate Change and Soif under Human Impact; International audience; The spatial organization of soil microbial communities over large areas and the identification of environmental factors structuring their distribution have been liUle investigated. The overall objective of this study was to determine the spatial paUerning of microbial biomass in soils on wide extent and to rank the environmental fiUers most influencing this distribution, by using the French Soil Quality Monitoring Network. This network covers ail the French terrilory and soils were sampled at2,150 sites along a systematic grid sampling. The soil DNA …
Inter-laboratory evaluation of the ISO standard 11063 "Soil quality -- Method to directly extract DNA from soil samples"
2011
International audience; Extracting DNA directly from micro-organisms living in soil is a crucial step for the molecular analysis of soil microbial communities. However, the use of a plethora of different soil DNA extraction protocols, each with its own bias, makes accurate data comparison difficult. To overcome this problem, a method for soil DNA extraction was proposed to the International Organization for Standardization (ISO) in 2006. This method was evaluated by 13 independent European laboratories actively participating in national and international ring tests. The reproducibility of the standardized method for molecular analyses was evaluated by comparing the amount of DNA extracted, …
Inter-laboratory evaluation of the ISO standard 11063 "Soil quality - Method to directly extract DNA from soil samples"
2011
International audience; Extracting DNA directly from micro-organisms living in soil is a crucial step for the molecular analysis of soil microbial communities. However, the use of a plethora of different soil DNA extraction protocols, each with its own bias, makes accurate data comparison difficult. To overcome this problem, a method for soil DNA extraction was proposed to the International Organization for Standardization (ISO) in 2006. This method was evaluated by 13 independent European laboratories actively participating in national and international ring tests. The reproducibility of the standardized method for molecular analyses was evaluated by comparing the amount of DNA extracted, …